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Particle tracking algorithms for cellular rheological property analysisTo adapt to the changes in the extracellular microenvironment, cells regulate their intracellular rheological properties to control their activities. Knowing the rheological properties therein let us be able to evaluate and elucidate their physiological and pathophysiological status. Particle tracking microrheology (PTM) is a technique to examine the intracellular rheological properties by measuring the trajectories of tracer particles. This can be performed in situ in an environment that cannot be reached by a conventional rheological experiment such as inside a living cell. Although tracking multiple particles with a microscope is convenient, analysis of the acquired video can be challenging. Typical steps involved in this analysis include (1) extracting the coordinates of all the particles in each frame of the video, (2) matching the particles frame-by-frame to produce their trajectories, and (3) computing the rheological properties based on the derived displacements. In this project, we are developing all the required algorithms to realize PTM.
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realization of a PTM system |
The following link provides the video for demonstrating our developed particle tracking microrheology system.
PTM System Demo |