Mesoscale neuronal ensemble data analysis for DrosophilA
The advance of two-photon microscopy (2PM) has made it possible to image few hundred micrometers deep in intact tissue with spatial resolution sufficient to discern individual neurons and to resolve the temporal evolution of the network states. While this imaging technique provides unprecedented insights into the brain network dynamics, it also poses increasing challenges for the analysis of the large volume of acquired data. The purpose of this project is to develop a systematical approach to 2PM data analysis. Topics to be covered include (1) individual analysis: automated functional area delineation and (2) group analysis: normalization, group functional area delineation, and neural network dynamics analysis. Furthermore, the application of these developed algorithms in investigating the formation of olfaction in Drosophila is also considered.
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